1KL7
Crystal Structure of Threonine Synthase from Yeast
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.92, 0.98, 0.9874 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.300, 51.600, 106.900 |
| Unit cell angles | 90.00, 99.60, 90.00 |
Refinement procedure
| Resolution | 8.000 * - 2.700 |
| R-factor | 0.212 * |
| Rwork | 0.201 |
| R-free | 0.25200 * |
| Structure solution method | MAD |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.350 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.063 | 0.172 |
| Total number of observations | 145806 * | |
| Number of reflections | 54312 * | |
| <I/σ(I)> | 16.8 | 4.6 |
| Completeness [%] | 98.8 | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 4 * | Hepes/NaOH, PEG4000, Dioxan, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | HEPES-NaOH | 0.1 (M) | pH7.6 |
| 3 | 1 | reservoir | PEG4000 | 20 (%) | |
| 4 | 1 | reservoir | dioxane | 10 (%) |
