1KK1
Structure of the large gamma subunit of initiation factor eIF2 from Pyrococcus abyssi-G235D mutant complexed with GDPNP-Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-10-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.934 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.164, 85.774, 57.377 |
Unit cell angles | 90.00, 109.28, 90.00 |
Refinement procedure
Resolution | 45.000 - 1.800 |
R-factor | 0.219 * |
Rwork | 0.219 |
R-free | 0.24500 |
Structure solution method | rigid body minimization |
Starting model (for MR) | 1kjz |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.043 * | 0.304 * |
Number of reflections | 44154 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 * | 297 | peg8000, peg8000, tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | 200 (mM) | ||
3 | 1 | drop | MOPS | 10 (mM) | pH6.7 |
4 | 1 | drop | 2-mercaptoethanol | 10 (mM) | |
5 | 1 | reservoir | PEG8000 | 8-18 (%) | |
6 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |