1KIC

Inosine-adenosine-guanosine preferring nucleoside hydrolase from Trypanosoma vivax: Asp10Ala mutant in complex with inosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF
Synchrotron site	ESRF
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-05
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9326
Spacegroup name	P 1 21 1
Unit cell lengths	54.350, 74.620, 73.020
Unit cell angles	90.00, 98.31, 90.00

Refinement procedure

Resolution	50.000 - 1.600
R-factor	0.1761 *
Rwork	0.176
R-free	0.19390 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hoz
RMSD bond length	0.016
RMSD bond angle	1.780
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.040	0.195 *
Number of reflections	76141
<I/σ(I)>	28.4	6.1
Completeness [%]	92.7 *	60.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	20 *	100 mM tris, 1.6 M ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	ammonium sulfate	1.6 (M)
3	1	reservoir	Tris	100 (mM)	pH8.5