1KF9

PHAGE DISPLAY DERIVED VARIANT OF HUMAN GROWTH HORMONE COMPLEXED WITH TWO COPIES OF THE EXTRACELLULAR DOMAIN OF ITS RECEPTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	193
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	67.289, 111.937, 95.290
Unit cell angles	90.00, 90.06, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.234
Rwork	0.234
R-free	0.32600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.800
Data reduction software	AUTOMAR
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.049	0.210
Total number of observations	215604 *
Number of reflections	43162
<I/σ(I)>	23	4.2
Completeness [%]	95.9	78.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.3		pH 6.30

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ammonium acetate	0.2 (M)
3	1	reservoir	sodium citrate	0.1 (M)
4	1	reservoir	PEG2500	30 (%)	pH6.3