1K9Z
The PAPase Hal2p complexed with zinc ions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-08-12 |
Detector | BRANDEIS |
Wavelength(s) | 0.9393 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.682, 45.359, 71.280 |
Unit cell angles | 90.00, 111.40, 90.00 |
Refinement procedure
Resolution | 65.940 - 1.500 |
R-factor | 0.16112 |
Rwork | 0.160 |
R-free | 0.19048 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qgx |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.740 | 1.539 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.090 | 0.430 |
Number of reflections | 50496 | |
<I/σ(I)> | 14.3 | 3.5 |
Completeness [%] | 96.4 | 96.5 |
Redundancy | 3.7 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 30 % PEG5000 MME, 0.1 M sodium acetate, 5 mM beta-mercaptoethanol, 0.1 M MES. (Crystals were soaked in mother liquor containing 0.5 M zinc chloride for 5 hours.) , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |