1K83

Crystal Structure of Yeast RNA Polymerase II Complexed with the Inhibitor Alpha Amanitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-11
Detector	ADSC QUANTUM 4
Spacegroup name	I 2 2 2
Unit cell lengths	122.510, 222.480, 374.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.229
Rwork	0.229
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i50
RMSD bond length	0.008 *
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.067	0.216
Number of reflections	124441	12292 *
Completeness [%]	99.8	99.4
Redundancy	3.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *		Fu, J., (1999) Cell, 98, 799. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-8 (mg)
2	1	reservoir	dioxane	50 (mM)
3	1	reservoir	dithiothreitol	10 (mM)
4	1	reservoir		390 (mM)	pH6.5
5	1	reservoir	PEG6000