1K5G
Crystal structure of Ran-GDP-AlFx-RanBP1-RanGAP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 99.930, 102.568, 118.850 |
| Unit cell angles | 71.67, 79.09, 67.81 |
Refinement procedure
| Resolution | 31.000 - 3.100 |
| R-factor | 0.25 * |
| Rwork | 0.250 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k5d |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.300 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.166 | 0.235 |
| Total number of observations | 207495 * | |
| Number of reflections | 92906 | |
| <I/σ(I)> | 3 | 1.7 |
| Completeness [%] | 96.0 | 95 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 20 * | PEG4000, TRIS-HCL, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 2 (mM) | ||
| 2 | 1 | drop | 30 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
| 4 | 1 | drop | 2 (mM) | ||
| 5 | 1 | drop | DTE | 2 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 18.5-20.5 (%) | |
| 7 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.5 |
