1K1D

Crystal structure of D-hydantoinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	125
Detector technology	CCD
Collection date	2001-01-07
Detector	CUSTOM-MADE
Wavelength(s)	0.91
Spacegroup name	C 1 2 1
Unit cell lengths	214.449, 182.890, 156.131
Unit cell angles	90.00, 125.27, 90.00

Refinement procedure

Resolution	20.000 * - 3.000 *
R-factor	0.263
Rwork	0.263
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	22.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE (& GLRF)
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.138 *
Total number of observations	943917 *
Number of reflections	96187
Completeness [%]	99.9	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	21 *	Ammonium Sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)	pH7.5
3	1	drop		200 (mM)
4	1	reservoir	Tris-Cl	100 (mM)	pH7.5
5	1	reservoir	ammonium sulfate	2.1 (M)