1K0N
Chloride Intracellular Channel 1 (CLIC1) complexed with glutathione
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-12-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.509, 60.355, 89.627 |
Unit cell angles | 90.00, 92.50, 90.00 |
Refinement procedure
Resolution | 91.290 - 1.800 |
R-factor | 0.253 * |
Rwork | 0.251 |
R-free | 0.28500 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 * |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.046 | 0.200 * |
Number of reflections | 43638 | |
<I/σ(I)> | 9.6 | 3.4 |
Completeness [%] | 98.0 | 93.9 |
Redundancy | 4.6 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 5 | 4 * | PEG MME 5K, AMMONIUM SULPHATE, SODIUM ACETATE, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-20 (mg/ml) | |
2 | 1 | drop | n-octyl-beta-D-glucopyranoside | 0.5 (%) | |
3 | 1 | reservoir | PEG5000 MME | 14-18 (%) | |
4 | 1 | reservoir | ammonium sulfate | 0-30 (mM) | |
5 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.0 |