1JX2
CRYSTAL STRUCTURE OF THE NUCLEOTIDE-FREE DYNAMIN A GTPASE DOMAIN, DETERMINED AS MYOSIN FUSION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2000-10-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.450, 62.040, 181.200 |
| Unit cell angles | 90.00, 94.79, 90.00 |
Refinement procedure
| Resolution | 14.960 - 2.300 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g8x MYOSIN II CATALYTIC DOMAIN |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 * | 0.215 * |
| Number of reflections | 52742 | |
| <I/σ(I)> | 19.3 | 4.98 |
| Completeness [%] | 97.9 | 94.6 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | PEG 8000, Tris, potassium chloride, magnesium chloride, glucose, methyl-propane-diol, dithiothreitol, EGTA, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 11 (%) | |
| 10 | 1 | drop | 1 (mM) | ||
| 11 | 1 | drop | dithiothreitol | 1 (mM) | |
| 12 | 1 | drop | sucrose | 3 (%) | |
| 13 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | Tris | 50 (mM) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | 5 (mM) | ||
| 5 | 1 | reservoir | glucose | 10 (%) | |
| 6 | 1 | reservoir | MPD | 2 (%) | |
| 7 | 1 | reservoir | EGTA | 1 (mM) | |
| 8 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 9 | 1 | drop | Tris | 50 (mM) |
