1JVA
CRYSTAL STRUCTURE OF THE VMA1-DERIVED ENDONUCLEASE BEARING THE N AND C EXTEIN PROPEPTIDES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 0.7000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.178, 68.846, 58.524 |
| Unit cell angles | 103.10, 98.79, 78.98 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vde |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.032 | 0.155 |
| Total number of observations | 122186 * | |
| Number of reflections | 51056 | 4038 * |
| <I/σ(I)> | 30.6 | 2.63 |
| Completeness [%] | 89.4 | 70.9 |
| Redundancy | 2.39 | 1.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 20 * | PEG6000, BisTrisHCl, mercaptoethanol, magnesium chloride, cadmium chloride, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG6000 | 17 (%(w/v)) | |
| 2 | 1 | reservoir | Bis-Tris-HCl | 0.1 (M) | pH6.2 |
| 3 | 1 | reservoir | 2-mercaptoethanol | 10 (mM) | |
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | reservoir | 1 (mM) |
