1JUV

Crystal structure analysis of Dihydrofolate reductase from Bacteriophage T4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Temperature [K]	100
Detector technology	DIFFRACTOMETER
Collection date	2000-01-01
Detector	WEISSENBERG
Wavelength(s)	1.0000
Spacegroup name	P 43 2 2
Unit cell lengths	61.160, 61.160, 124.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.570 - 1.700
R-factor	0.248
Rwork	0.227
R-free	0.26900
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.068
Number of reflections	26714
Completeness [%]	99.7	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.1	295	NaCl, (NH4)2HPO4 , pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 295K