1JTP

Degenerate interfaces in antigen-antibody complexes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-08-01
Detector	MARRESEARCH
Wavelength(s)	0.98089
Spacegroup name	P 21 21 21
Unit cell lengths	67.123, 69.375, 113.231
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.000 - 1.900
Rwork	0.184
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB code: 1MEL
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.061	0.417
Number of reflections	40619
<I/σ(I)>	11.4	2
Completeness [%]	95.7	92
Redundancy	2.7	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	300	2M Na Formate, 100mM Na Citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K