1JR3
Crystal Structure of the Processivity Clamp Loader Gamma Complex of E. coli DNA Polymerase III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2000-10-17 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.698, 95.857, 285.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 500.000 - 2.700 |
| R-factor | 0.268 * |
| Rwork | 0.268 |
| R-free | 0.30430 |
| Structure solution method | MIR |
| RMSD bond length | 0.001 * |
| RMSD bond angle | 1.720 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 1000.000 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.136 * | |
| Total number of observations | 552088 * | |
| Number of reflections | 60657 | |
| Completeness [%] | 83.0 | 93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | 294 | Polyethylene glycol, ammonium sulphate, dithiothreitol, dimethylacetamide, hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 21K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Tris-HCl | 20 (mM) | |
| 2 | 1 | 1 | dithiothreitol | 2 (mM) | |
| 3 | 1 | 1 | protein | 100 (mg/ml) | |
| 4 | 1 | 2 | HEPES | 50 (mM) | |
| 5 | 1 | 2 | ammonium sulfate | 1.05-1.15 (M) | |
| 6 | 1 | 2 | PEG400 | 6-7.5 (%) | |
| 7 | 1 | 2 | N,N-dimethylacetamide | 320 (mM) | |
| 8 | 1 | 2 | dithiothreitol | 4 (mM) |
