1JOL

THE CRYSTAL STRUCTURE OF THE BINARY COMPLEX BETWEEN FOLINIC ACID (LEUCOVORIN) AND E. COLI DIHYDROFOLATE REDUCTASE

Experimental procedure

Spacegroup name	P 61
Unit cell lengths	92.577, 92.577, 73.132
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 1.900 *
R-factor	0.142
RMSD bond length	0.018
RMSD bond angle	2.967
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	1.900 *
Rmerge	0.058 *
Number of reflections	25677 *
Completeness [%]	100.0 *
Redundancy	6.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	L-histidine	0.5 (M)	0.0625 ml
2	1	drop	calcium acetate	0.5 (M)	0.0625 ml
3	1	drop	concentrated folnic acid-DHFR complex		0.05 ml
4	1	reservoir	ethanol	18-25 (%)
5	1	reservoir	Tris-HCl	1.0 (M)