1JLE

CRYSTAL STRUCTURE OF Y188C MUTANT HIV-1 REVERSE TRANSCRIPTASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID2
Synchrotron site	ESRF
Beamline	ID2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-06-27
Detector	MAR scanner 345 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	137.800, 109.500, 72.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.630 - 2.800
R-factor	0.261
Rwork	0.261
R-free	0.33700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.073
Number of reflections	26234
<I/σ(I)>	14.1	1.6
Completeness [%]	94.1	81.9
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5		pH 5.00