1JL9
Crystal Structure of Human Epidermal Growth Factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Synchrotron site | APS |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 61.430, 61.430, 87.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 3.000 |
| R-factor | 0.231 * |
| Rwork | 0.231 |
| R-free | 0.28300 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.193 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | DM |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.104 | |
| Total number of observations | 21097 * | |
| Number of reflections | 4040 | |
| Completeness [%] | 99.0 | 96.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 291 * | magnesium chloride, Bicine, CYMAL-3, sodium azide, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 0.9 (M) | ||
| 2 | 1 | reservoir | CYMAL-3 | 3.5 (mM) | |
| 3 | 1 | reservoir | Bicine | 0.1 (M) | |
| 4 | 1 | drop | protein | 50 (mg/ml) |
