1JD2
Crystal Structure of the yeast 20S Proteasome:TMC-95A complex: A non-covalent Proteasome Inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-05-12 |
Detector | MARRESEARCH |
Wavelength(s) | 1.050 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 135.200, 301.300, 144.800 |
Unit cell angles | 90.00, 112.60, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.000 |
R-factor | 0.271 |
Rwork | 0.251 |
R-free | 0.33600 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.013 |
RMSD bond angle | 1.987 |
Data reduction software | TRUNCATE |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.000 |
Rmerge | 0.124 |
Total number of observations | 741075 * |
Number of reflections | 255383 * |
<I/σ(I)> | 4.22 |
Completeness [%] | 98.4 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 24 * | MPD, MES, DMSO, magnesium Acetate, pH 6.4, VAPOR DIFFUSION, HANGING DROP at 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 40 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | reservoir | magnesium acetate | 30 (mM) | |
5 | 1 | reservoir | MES | 100 (mM) | pH7.2 |
6 | 1 | reservoir | MPD | 10 (%(v/v)) |