1JCA
Non-standard Design of Unstable Insulin Analogues with Enhanced Activity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 290 |
Detector technology | IMAGE PLATE |
Collection date | 2000-01-01 |
Detector | RIGAKU RAXIS II |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 |
Unit cell lengths | 80.652, 80.652, 37.936 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.500 |
R-factor | 0.204 * |
Rwork | 0.204 |
R-free | 0.24100 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1trz |
RMSD bond length | 0.009 |
RMSD bond angle | 23.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.076 * | 0.160 |
Number of reflections | 3169 | |
Completeness [%] | 99.2 | 97.5 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 * | Tris, sodium citrate, acetone, phenol, pH 7.0, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | 0.02 (M) | ||
3 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
4 | 1 | reservoir | sodium citrate | 0.1 (M) | pH7.8 |
5 | 1 | reservoir | zinc acetate | 0.02 (%) | |
6 | 1 | reservoir | acetate | 8 (%) | |
7 | 1 | reservoir | phenol | 0.5 (%) |