1J2F
X-ray crystal structure of IRF-3 and its functional implications
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-25 |
| Detector | OXFORD PX210 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 134.808, 134.808, 69.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 500.000 * - 2.300 |
| R-factor | 0.2194 |
| Rwork | 0.217 |
| R-free | 0.24200 * |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.260 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.017 | 2.110 |
| High resolution limit [Å] | 2.300 * | 1.999 |
| Rmerge | 0.094 * | |
| Number of reflections | 24036 * | |
| Completeness [%] | 94.6 * | 95.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 * | 4 * | magnesium formate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH7.9 |
| 3 | 1 | drop | 500 (mM) | ||
| 4 | 1 | reservoir | sodium acetate | 100 (mM) | pH5.1 |
| 5 | 1 | reservoir | magnesium formate | 400 (mM) |
