1IVO

Crystal Structure of the Complex of Human Epidermal Growth Factor and Receptor Extracellular Domains.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Wavelength(s)	1.00
Spacegroup name	P 31 2 1
Unit cell lengths	220.164, 220.164, 113.121
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 3.300
R-factor	0.258
Rwork	0.255
R-free	0.32600
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.390 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.710
High resolution limit [Å]	3.300 *	3.500
Rmerge	0.078 *	0.358 *
Total number of observations	320883 *
Number of reflections	46667 *
<I/σ(I)>	25.6	5.1
Completeness [%]	98.2 *	92.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	20 *	PEG4000, PEG6000, sodium acetate, sodium chloride, TRIS, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8.4	20 *	PEG4000, PEG6000, sodium acetate, sodium chloride, TRIS, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	15 (%)
2	1	reservoir	PEG6000	1 (%)
3	1	reservoir	Tris-HCl	75 (mM)	pH8.4
4	1	reservoir	sodium acetate	75 (mM)
5	1	reservoir		200 (mM)
6	1	drop	protein	10 (mg/ml)