1INR

CYTOKINE SYNTHESIS

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1994-09
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	36.370, 36.370, 220.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.221
Rwork	0.221
R-free	0.33700
RMSD bond length	0.020
RMSD bond angle	21.840 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.000
Rmerge	0.080
Total number of observations	42251 *
Number of reflections	10087
Completeness [%]	92.0 *
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	35 (%sat)
2	1	1	HEPES	100 (mM)