1ILE
ISOLEUCYL-TRNA SYNTHETASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-05 |
| Detector | RIGAKU |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.100, 94.800, 126.500 |
| Unit cell angles | 90.00, 126.30, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.29800 |
| Structure solution method | MIR |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.650 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.064 | |
| Total number of observations | 134577 * | |
| Number of reflections | 48290 | |
| <I/σ(I)> | 8.14 | |
| Completeness [%] | 91.0 | 76.3 |
| Redundancy | 3.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 20 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 12 (%) | |
| 3 | 1 | reservoir | isopropanol | 6 (%) | |
| 4 | 1 | reservoir | MPD | 1 (%) | |
| 5 | 1 | reservoir | HEPES | 60 (mM) |
