1IIU

Chicken plasma retinol-binding protein (RBP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER
Temperature [K]	293
Detector technology	AREA DETECTOR
Detector	SIEMENS HI-STAR
Spacegroup name	P 21 21 21
Unit cell lengths	46.064, 53.560, 73.414
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.280 - 2.500
R-factor	0.21 *
Rwork	0.211
R-free	0.26000 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016 *
RMSD bond angle	2.800 *
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	AMoRE
Refinement software	CNX (2000.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.500	2.500 *
Rmerge	0.120 *	0.320 *
Total number of observations	22515 *
Number of reflections	6664 *	680 *
Completeness [%]	87.8 *	68 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6 *	4 *	pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	MPD	8 (%(v/v))
3	1	reservoir	cadmium acetate	8 (mM)
4	1	reservoir	sodium acetate	20 (mM)
5	1	reservoir	sodium cacodylate	40 (mM)	pH6.6