1IIP
Bovine Cyclophilin 40, Tetragonal Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-06-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 94.585, 94.585, 118.326 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.000 - 2.000 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.30500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CYCLOPHILIN A |
| RMSD bond length | 0.060 * |
| RMSD bond angle | 0.220 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.057 | 0.325 |
| Number of reflections | 33904 * | |
| <I/σ(I)> | 19.65 | 3.15 |
| Completeness [%] | 90.5 | 90.5 |
| Redundancy | 3.62 | 3.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 4 * | MPD, Imidazole glycerol Tris Sodium Chloride, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 0.480ml | |
| 2 | 1 | reservoir | imidazole | 1 (M) | 0.100ml |
| 3 | 1 | reservoir | glycerol | 50 (%(v/v)) | 0.140ml |
| 4 | 1 | reservoir | water | 0.280ml | |
| 5 | 1 | drop | protein | 10-80 (mg/ml) | |
| 6 | 1 | drop | Tris | 20 (mM) | |
| 7 | 1 | drop | 100 (mM) |
