1IIK
CRYSTAL STRUCTURE OF THE TRANSTHYRETIN MUTANT TTR Y114C-DATA COLLECTED AT CRYO TEMPERATURE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-30 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.288, 85.288, 64.781 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.300 * - 2.000 |
| R-factor | 0.1969 |
| Rwork | 0.192 |
| R-free | 0.23900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1f41 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.260 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.077 | 0.272 |
| Total number of observations | 102762 * | |
| Number of reflections | 16411 | 1560 * |
| <I/σ(I)> | 9.5 | 4.1 |
| Completeness [%] | 96.9 | 94.8 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 100 * | 40% PEG 550 monomethyl ether, 0.1M sodium citrate, 0.1M ammonium sulphate, 0.1% BME, pH 5.0, VAPOR DIFFUSION, HANGING DROP at 298K, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH7.5 |
| 3 | 1 | reservoir | PEG550 MME | 36-40 (%) | |
| 4 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.0 |
| 5 | 1 | reservoir | ammonium sulfate | 100 (mM) | |
| 6 | 1 | reservoir | beta-mercaptoethanol | 0.1 (%) |
