1IGT

STRUCTURE OF IMMUNOGLOBULIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	291
Detector technology	AREA DETECTOR
Collection date	1992-11
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 1
Unit cell lengths	65.820, 76.770, 100.640
Unit cell angles	88.05, 92.35, 97.23

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.209
Rwork	0.209
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	VL:VH DOMAIN PAIR OF PDB ENTRY 1MCP CL:CH1 DOMAIN PAIR OF ENTRY 2HFL FC FRAGMENT OF ENTRY 1FC1
RMSD bond length	0.009
RMSD bond angle	27.100 *
Data reduction software	HOWARD
Data scaling software	XENGEN ((HOWARD)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	76.100
High resolution limit [Å]	2.800
Rmerge	0.100 *
Total number of observations	200000 *
Number of reflections	47595
<I/σ(I)>	7.1
Completeness [%]	99.0
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8	18 *	Larson, S., (1991) J. Mol. Biol., 222, 17. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.1 (mg/ml)
2	1	drop	Tris-HCl	0.01 (M)
3	1	drop	PEG3350	1.8 (%)
4	1	reservoir	PEG3350	4 (%)