1IE4
RAT TRANSTHYRETIN COMPLEX WITH THYROXINE (T4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-10-01 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 82.526, 82.526, 161.844 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.500 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.27500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1gke |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.280 * |
| Phasing software | X-PLOR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.500 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.106 | 0.350 |
| Total number of observations | 83761 * | |
| Number of reflections | 16558 * | |
| <I/σ(I)> | 6 | 2.19 |
| Completeness [%] | 83.5 * | 82.2 |
| Redundancy | 4.4 | 2.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | Ammonium sulfate, acetate buffer, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 60 (%) | |
| 2 | 1 | reservoir | acetate | 0.1 (M) | pH5.0 |
| 3 | 1 | drop | ammonium sulfate | 55-65 (%) | |
| 4 | 1 | drop | acetate | 0.1 (M) | pH5.0 |
