1IE4

RAT TRANSTHYRETIN COMPLEX WITH THYROXINE (T4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1994-10-01
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	82.526, 82.526, 161.844
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 2.500
R-factor	0.209
Rwork	0.209
R-free	0.27500
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1gke
RMSD bond length	0.007 *
RMSD bond angle	1.280 *
Phasing software	X-PLOR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.500	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.106	0.350
Total number of observations	83761 *
Number of reflections	16558 *
<I/σ(I)>	6	2.19
Completeness [%]	83.5 *	82.2
Redundancy	4.4	2.14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	Ammonium sulfate, acetate buffer, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	60 (%)
2	1	reservoir	acetate	0.1 (M)	pH5.0
3	1	drop	ammonium sulfate	55-65 (%)
4	1	drop	acetate	0.1 (M)	pH5.0