1I1R
CRYSTAL STRUCTURE OF A CYTOKINE/RECEPTOR COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.000, 123.310, 76.790 |
| Unit cell angles | 90.00, 112.03, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | human Interleukin-6 1ALU gp130 D2D3 domains 1BQU |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.600 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.081 | 0.487 |
| Total number of observations | 106397 * | |
| Number of reflections | 34376 | |
| <I/σ(I)> | 3.4 | 1.4 |
| Completeness [%] | 99.0 | 99.6 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | 293 | MPEG 2000, sodium citrate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 2 | 1 | 1 | mPEG2000 | 10 (%) | |
| 3 | 1 | 1 | Na citrate | 0.1 (M) |
