1I1C
NON-FCRN BINDING FC FRAGMENT OF RAT IGG2A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-08-02 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.503, 73.362, 82.157 |
| Unit cell angles | 90.00, 102.74, 90.00 |
Refinement procedure
| Resolution | 29.810 - 2.700 |
| R-factor | 0.241 * |
| Rwork | 0.241 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fc1 |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.068 | 0.261 * |
| Total number of observations | 35621 * | |
| Number of reflections | 14919 * | 1323 * |
| <I/σ(I)> | 13.4 | 3.6 |
| Completeness [%] | 97.5 * | 87.2 |
| Redundancy | 1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 * | 298 | PEG 4000 ammonium acetate tri-sodium acetate dihydrate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 2 | 1 | reservoir | trisodium acetate dihydrate | 0.1 (M) | |
| 3 | 1 | reservoir | PEG4000 | 30 (%) |
