1HX0

Structure of pig pancreatic alpha-amylase complexed with the "truncate" acarbose molecule (pseudotrisaccharide)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-11-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 21 21 21
Unit cell lengths	69.280, 113.550, 117.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 1.380
R-factor	0.1083
Rwork	0.108
R-free	0.13020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ppi
RMSD bond length	0.013 *
RMSD bond angle	0.030 *
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.420
High resolution limit [Å]	1.380	1.380
Rmerge	0.067	0.451 *
Total number of observations	507677 *
Number of reflections	184003
<I/σ(I)>	13.1	1.93
Completeness [%]	97.0	96.13
Redundancy	2.8	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	batch	8.5	277	Qian, M., (1993) J. Mol. Biol., 232, 785. *