1HWY

BOVINE GLUTAMATE DEHYDROGENASE COMPLEXED WITH NAD AND 2-OXOGLUTARATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	122.500, 101.000, 164.600
Unit cell angles	90.00, 102.20, 90.00

Refinement procedure

Resolution	8.000 - 3.200
Rwork	0.230
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010 *
RMSD bond angle	1.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.400
High resolution limit [Å]	3.000	3.200
Rmerge	0.123 *	0.475 *
Completeness [%]	79.0 *	80 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	sodium phosphate, NaCl, PEG 8000, sodium azide, methyl pentanediol, octyl-b-glucopyranoside, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	NAD+	1.5 (mM)
2	1	drop	alpha-KG	20 (mM)
3	1	drop	boGDH	3.25 (mg/ml)
4	1	reservoir	sodium phosphate	0.1 (M)
5	1	reservoir	octyl-beta-glucopyranoside	1 (%)
6	1	reservoir	methyl pentanediol	5 (%)
7	1	reservoir	sodium chloride	0.5 (M)
8	1	reservoir	sodium azide	1 (mM)