1HWW

GOLGI ALPHA-MANNOSIDASE II IN COMPLEX WITH SWAINSONINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-04-25
Detector	MARRESEARCH
Wavelength(s)	1.54189
Spacegroup name	P 21 21 21
Unit cell lengths	68.902, 110.015, 138.472
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 * - 1.870
R-factor	0.181 *
Rwork	0.181
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hty
RMSD bond length	0.005
RMSD bond angle	1.310 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.240	1.910
High resolution limit [Å]	1.870	1.870
Rmerge	0.078	0.452
Number of reflections	87386 *	5601 *
<I/σ(I)>	24	3.8
Completeness [%]	99.7	96.9
Redundancy	6.9	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	298	PEG 6000, MPD, Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	8.5 (%)
2	1	reservoir	MPD	5 (%)
3	1	reservoir	Tris	100 (mM)	pH7.0