1HW1
THE FADR-DNA COMPLEX: TRANSCRIPTIONAL CONTROL OF FATTY ACID METABOLISM IN ESCHERICHIA COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-02 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.346, 86.996, 59.128 |
| Unit cell angles | 90.00, 120.18, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.500 |
| R-factor | 0.198 * |
| Rwork | 0.198 |
| R-free | 0.21600 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 * |
| RMSD bond angle | 1.109 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | |
| High resolution limit [Å] | 1.500 | |
| Rmerge | 0.067 | 0.180 * |
| Total number of observations | 1306223 * | |
| Number of reflections | 80658 | |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 97.2 | 78.9 * |
| Redundancy | 16.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | 18 * | ammonium sulfate, lithium sulfate, sodium citrate, zinc chloride, pH 5.6, EVAPORATION, temperature 290.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
| 3 | 1 | reservoir | ammonium sulfate | 0.7 (M) | |
| 4 | 1 | reservoir | lithium sulfate | 0.2 (M) | |
| 5 | 1 | reservoir | zinc chloride | 5 (mM) |
