1HUW
THE CRYSTAL STRUCTURE OF AFFINITY-MATURED HUMAN GROWTH HORMONE AT 2 ANGSTROMS RESOLUTION
Experimental procedure
Spacegroup name | C 1 2 1 |
Unit cell lengths | 80.450, 59.340, 50.870 |
Unit cell angles | 90.00, 128.20, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.400 * |
R-factor | 0.185 |
Rwork | 0.185 |
RMSD bond length | 0.012 |
RMSD bond angle | 2.630 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 8.000 * | |
High resolution limit [Å] | 2.400 * | 2.400 * |
Rmerge | 0.069 * | 0.144 * |
Total number of observations | 16335 * | |
Number of reflections | 6630 * | 626 * |
Completeness [%] | 97.3 * | 94.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 10 (%(w/v)) | |
10 | 1 | drop | magnesium acetate | 0.2 (M) | |
11 | 1 | drop | sodium cacodylate | 0.1 (M) | |
2 | 1 | reservoir | magnesium acetate | 0.2 (M) | |
3 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
4 | 1 | drop | protein | 6 (mg/ml) | |
5 | 1 | drop | 120 (mM) | ||
6 | 1 | drop | sodium cacoylate | 20 (mM) | |
7 | 1 | drop | EDTA | 5.0 (mM) | |
8 | 1 | drop | phenylmethylsulfonyl fluoride | 1 (mM) | |
9 | 1 | drop | PEG8000 | 10 (%(w/v)) |