1HUT
THE STRUCTURE OF ALPHA-THROMBIN INHIBITED BY A 15-MER SINGLE-STRANDED DNA APTAMER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.520, 77.440, 99.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.900 |
| R-factor | 0.159 |
| Rwork | 0.159 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 3.300 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.870 | 2.870 * |
| Total number of observations | 12408 * | |
| Number of reflections | 6990 | |
| Completeness [%] | 67.0 * | 32 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.3 * | Skrzypczak, J., (1991) J. Mol. Biol., 221, 1379. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.0-3.7 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 0.05 (M) | |
| 3 | 1 | drop | 0.375 (M) | ||
| 4 | 1 | drop | 0.5 (mM) | ||
| 5 | 1 | reservoir | sodium phospahte | 0.1 (M) | |
| 6 | 1 | reservoir | 1-2 (mM) | ||
| 7 | 1 | reservoir | PEG8000 | 25-30 (%(v/v)) |
