1HPL

HORSE PANCREATIC LIPASE. THE CRYSTAL STRUCTURE AT 2.3 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	79.800, 97.200, 145.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.300
R-factor	0.159
Rwork	0.159
RMSD bond length	0.015
RMSD bond angle	3.100
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.300 *	2.300 *
Rmerge	0.063 *
Total number of observations	145844 *
Number of reflections	45644 *
Completeness [%]	86.3 *	48 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6 *	20 *	referred to J.Mol.Biol. 205.259-261 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	8 (mg/ml)
2	1	2	MES	0.1 (M)
3	1	2	PEG8000	15-20 (%(w/v))
4	1	2		10 (mM)
5	1	2		0.1 (M)