1HLZ
CRYSTAL STRUCTURE OF THE ORPHAN NUCLEAR RECEPTOR REV-ERB(ALPHA) DNA-BINDING DOMAIN BOUND TO ITS COGNATE RESPONSE ELEMENT
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04-05 |
| Detector | FUJI |
| Wavelength(s) | 0.92016 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.867, 39.603, 58.565 |
| Unit cell angles | 84.13, 72.09, 70.20 |
Refinement procedure
| Resolution | 18.600 - 2.800 |
| R-factor | 0.2599 |
| Rwork | 0.260 |
| R-free | 0.31030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a6y residues 132-198 from chain A & B plus DNA |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 2.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.620 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.078 * | 0.258 * |
| Number of reflections | 6854 * | 732 * |
| <I/σ(I)> | 9 | 2.1 |
| Completeness [%] | 96.6 * | 93.3 * |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 17 * | Zhao, Q., (1998) Mol. Cell, 1, 849. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG8000 | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | Tris |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.6 (mM) | |
| 2 | 1 | drop | protein | 1.2 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 20-25 (%) | |
| 4 | 1 | reservoir | 5 (mM) | ||
| 5 | 1 | reservoir | 400 (mM) | ||
| 6 | 1 | reservoir | Tris | pH7.5 |
