1HJX
Ligand-induced signalling and conformational change of the 39 kD glycoprotein from human articular chondrocytes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2002-09-15 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 109.562, 122.048, 136.097 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.980 - 1.850 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lg2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.076 | 0.519 |
| Number of reflections | 153614 | |
| <I/σ(I)> | 9.5 | 3.7 |
| Completeness [%] | 99.0 | 98.4 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.2 M AMMONIUM SULFATE, 0.1 M SODIUM CITRATE PH 4.6, 25% PEG 4000, 100 MM DTT |
