1HC7
Prolyl-tRNA synthetase from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-04-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 132.300, 191.100, 125.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.430 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.23400 |
Structure solution method | SIRAS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.286 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | RSPS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.490 |
High resolution limit [Å] | 2.430 | 2.430 |
Rmerge | 0.053 | 0.244 |
Number of reflections | 117904 | |
<I/σ(I)> | 2.9 | |
Completeness [%] | 98.6 | 93.1 |
Redundancy | 3.8 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.9 | SEE REFERENCE 2, pH 7.90 |