1HBZ

Catalase from Micrococcus lysodeikticu

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	287
Spacegroup name	P 42 21 2
Unit cell lengths	106.700, 106.700, 106.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 1.500
Rwork	0.092
R-free	0.12000
Structure solution method	MIR
RMSD bond length	0.013
RMSD bond angle	0.001 *
Data scaling software	MOSFLM
Refinement software	REFMAC (5.0.36)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.000	1.540
High resolution limit [Å]	1.501	1.500
Rmerge	0.060	0.210
Total number of observations	358628 *
Number of reflections	95355 *
<I/σ(I)>	7.9	3.6
Completeness [%]	97.0	96
Redundancy	3.7	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.2 *		pH 6.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-30 (mg/ml)
2	1	drop	ammonium sulfate	0.6-0.8 (M)
3	1	drop	sodium acetate	0.05 (M)
4	1	reservoir	ammonium sulfate	1.2-1.4 (M)