1H6R

The oxidized state of a redox sensitive variant of green fluorescent protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-02-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	57.864, 93.915, 140.551
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.090 - 1.500
R-factor	0.183
Rwork	0.183
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yfp
RMSD bond length	0.011
RMSD bond angle	27.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.090	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.033	0.439
Total number of observations	750960 *
Number of reflections	116321
<I/σ(I)>	18.3	2
Completeness [%]	89.1	72.9
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8	22 *	100 MM HEPES, PH 8.0, 100 MM MGCL2, AND 14% PEG4000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	24 (mg/ml)
2	1	drop	HEPES	10 (mM)	pH8.0
3	1	reservoir	HEPES	100 (mM)	pH8.0
4	1	reservoir		100 (mM)
5	1	reservoir	PEG4000	14 (%(w/v))