1H2U

Structure of the human nuclear cap-binding-complex (CBC) in complex with a cap analogue m7GpppG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-18
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	111.838, 125.720, 188.756
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 2.400
R-factor	0.194
Rwork	0.194
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h6k
RMSD bond length	0.006
RMSD bond angle	20.300 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.360
High resolution limit [Å]	2.400 *	2.300
Rmerge	0.114	0.452
Number of reflections	91470 *
<I/σ(I)>	5.3	1.6
Completeness [%]	89.1	65.4
Redundancy	3.2	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6	277 *	Mazza, C., (2002) Acta Crystallogr.,Sect.D, 58, 2194. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG6000	10 (%)
3	1	reservoir	MES	100 (mM)	pH6.