1H2U
Structure of the human nuclear cap-binding-complex (CBC) in complex with a cap analogue m7GpppG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-18 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 111.838, 125.720, 188.756 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.400 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h6k |
| RMSD bond length | 0.006 |
| RMSD bond angle | 20.300 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.360 |
| High resolution limit [Å] | 2.400 * | 2.300 |
| Rmerge | 0.114 | 0.452 |
| Number of reflections | 91470 * | |
| <I/σ(I)> | 5.3 | 1.6 |
| Completeness [%] | 89.1 | 65.4 |
| Redundancy | 3.2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 277 * | Mazza, C., (2002) Acta Crystallogr.,Sect.D, 58, 2194. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG6000 | 10 (%) | |
| 3 | 1 | reservoir | MES | 100 (mM) | pH6. |
