1GZK

Molecular mechanism for the regulation of protein kinase B/Akt by hydrophobic motif phosphorylation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-09-15
Detector	ADSC CCD
Spacegroup name	P 41 21 2
Unit cell lengths	148.405, 148.405, 38.547
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 * - 2.300
R-factor	0.248
Rwork	0.248
R-free	0.30900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cdk
RMSD bond length	0.011 *
RMSD bond angle	1.540 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000
High resolution limit [Å]	2.300
Rmerge	0.050	0.243 *
Total number of observations	113677 *
Number of reflections	18905 *
<I/σ(I)>	21
Completeness [%]	96.1	77.8 *
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.5	20 *	10MG/ML PROTEIN, 30% PEG4K, 0.2 M LITHIUM SULPHATE, 0.1 M TRIS, pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	1	AMP-PNP-MgCl2	5 (mM)
3	1	1	PEG400	30 (%(w/v))
4	1	1	lithium sulfate	0.2 (M)
5	1	1	Tris-HCl	0.1 (M)	pH8.5
6	1	1	dithiothreitol	5 (mM)