1GZH

Crystal structure of the BRCT domains of human 53BP1 bound to the p53 tumor supressor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-05-15
Detector	MAR scanner 345 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	71.520, 94.570, 136.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 * - 2.600
R-factor	0.238
Rwork	0.238
R-free	0.28830 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tsr
RMSD bond length	0.008
RMSD bond angle	1.349 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000 *	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.104	0.358
Number of reflections	54119
<I/σ(I)>	6.2	1.4
Completeness [%]	99.1	96.9
Redundancy	7.7	3.70 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.4		50 MM TRIS PH 7.4, 250 MM AMMONIUM SULFATE, 25% POLYETHYLENE GLYCOL 4000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	15 (mg/ml)
2	1	1	Tris-HCl	50 (mM)	pH7.4
3	1	1	ammonium sulfate
4	1	1	PEG4000	20-25 (%)