1GZ5
Trehalose-6-phosphate synthase. OtsA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-15 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 103.612, 125.400, 176.607 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.000 - 2.430 |
R-factor | 0.207 |
Rwork | 0.206 |
R-free | 0.24000 * |
Structure solution method | MAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.950 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.540 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.056 | 0.430 * |
Number of reflections | 86205 | |
<I/σ(I)> | 17 | 2.4 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 2.8 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7.5 | 14% PEG4000, 0.15M NAAC, 0.1M IMIDAZOLE PH 7.5, 10MM MG-UDP, 10MM G6P |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | PEG4000 | 14 (%(v/v)) | |
2 | 1 | 1 | 0.15 (M) | ||
3 | 1 | 1 | imidazole | 0.1 (M) | pH7.5 |
4 | 1 | 1 | 200 (mM) |