1GY8
Trypanosoma brucei UDP-galactose 4' epimerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 102.185, 112.533, 160.591 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.000 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ek6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.284 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 | 0.361 |
| Total number of observations | 417521 * | |
| Number of reflections | 112132 | |
| <I/σ(I)> | 9.09 | 1.15 |
| Completeness [%] | 90.4 | 59.3 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | PROTEIN WAS CRYSTALLISED WITH NAD AND UDP, FROM 100M HEPES PH7.0, 14% PEG8000, 200MM KCL, pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 20 (mg/ml) | |
| 2 | 1 | drop | NAD+ | 5 (mM) | |
| 3 | 1 | drop | UDP | 5 (mM) | |
| 4 | 1 | drop | Tris-HCl | 50 (mM) | pH7.5 |
| 5 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
| 6 | 1 | reservoir | PEG8000 | 14 (%) | |
| 7 | 1 | reservoir | 200 (mM) | ||
| 8 | 1 | reservoir | PEG400 | 3 (%) |
