1GXD

proMMP-2/TIMP-2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-05-15
Spacegroup name	C 2 2 21
Unit cell lengths	75.696, 374.575, 191.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.940 * - 3.100
R-factor	0.278
Rwork	0.275
R-free	0.33500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ck7 1br9
RMSD bond length	0.009 *
RMSD bond angle	1.639 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BLANC
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.940 *
High resolution limit [Å]	3.100	3.100 *
Rmerge	0.110 *
Total number of observations	475848 *
Number of reflections	46264
<I/σ(I)>	10
Completeness [%]	94.1	70.8 *
Redundancy	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.25 *	4 *	pH 8.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	11 (mg/ml)
2	1	reservoir		0.2 (M)
3	1	reservoir	reduced	0.2 (mM)
4	1	reservoir	oxidized glutathione	0.2 (mM)
5	1	reservoir	PEG4000	4 (%)
6	1	reservoir	ammonium acetate	0.1 (M)	pH6.25