1GX5
Hepatitis C Virus RNA Polymerase in Complex with GTP and Manganese
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 67.076, 96.225, 95.995 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.780 - 1.700 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1csj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 22.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.069 | 0.423 |
| Number of reflections | 68538 | |
| <I/σ(I)> | 12.3 | 2.9 |
| Completeness [%] | 99.2 | 98.5 * |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 4 * | Bressanelli, S., (1999) Proc. Natl. Acad. Sci. U.S.A., 96, 13034. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | dithiothreitol | 20 (mM) | |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 5 (%(w/v)) | |
| 6 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 7 | 1 | reservoir | 2-propanol | 5 (%(v/v)) |
