1GT0

Crystal structure of a POU/HMG/DNA ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-15
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	72.800, 72.800, 172.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.600
R-factor	0.232
Rwork	0.232
R-free	0.28500
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.047	0.350
Number of reflections	16619
<I/σ(I)>	21.8	2.5
Completeness [%]	98.3	96.5
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		18% PEG3350, 50 MM HEPES PH 7.0, 20 MM MGCL2, 5% GLYCEROL